Journal: Pathogens

Article Title: Optimizing Photosensitizer Delivery for Effective Photodynamic Inactivation of Klebsiella pneumoniae Under Lung Surfactant Conditions

doi: 10.3390/pathogens14070618

Figure Lengend Snippet: ( a ) Antimicrobial effect of Gantrez AN-139 at different concentrations after irradiation (150 J/cm 2 ); ( b ) aPDT using 25 µg/mL of ICG + 0.5% ( w / v ) Gantrez AN-139 in a medium containing 5% ( v / v ) dipalmitoylphosphatidylcholine (DPPC) (150 J/cm 2 ) ( n = 4). * p < 0.05; *** p < 0.001; **** p < 0.0001; ns: not significant ( p > 0.05).

Article Snippet: Then, to evaluate the inhibitory effect of LS on aPDT, its main component, dipalmitoylphosphatidylcholine (DPPC, Chem-Impex, Wood Dale, IL, USA), was prepared in PBS at a concentration of 30 mg/mL.

Techniques: Irradiation

Journal: The Journal of Clinical Investigation

Article Title: Peroxisome-derived lipids regulate adipose thermogenesis by mediating cold-induced mitochondrial fission

doi: 10.1172/JCI120606

Figure Lengend Snippet: (A) Ether lipid synthetic pathway. The initial steps for synthesis of ether lipids, including plasmalogens, take place in peroxisomes, generating 1-O-alkyl-glycerol-3-phosphate (AGP), a precursor for ether-linked analogs of PC and phosphatidylethanolamine. DHAP, dihydroxyacetone phosphate. (B and C) Western blot analysis suggesting that ether lipid synthetic enzymes are degraded in BAT (B) and iWAT (C) of Pex16-AKO mice (D) Targeted lipidomics analysis of mitochondrial phospholipids in BAT of WT C57 mice. PS, phosphatidylserine; PG, phosphatidylglycerol; aPC, alkyl ether PC; pPC, plasmalogen PC; PE, phosphatidylethanolamine; pPE, plasmalogen PE; n = 5. (E) Levels of various plasmalogen PE species in the mitochondrial fraction of BAT; n = 9–10. (F) Total diacyl and plasmalogen PE content in BAT mitochondria. (G) BAT SVF cells stably expressing Mito-roGFP were differentiated into adipocytes and then treated with scrambled or GNPAT shRNA and analyzed 5 days later for mitochondrial morphology using confocal microscopy. Images are representative of 3 separate experiments. Original magnification, ×60. (H) Differentiated BAT SVF cells were treated with scrambled or GNPAT shRNA. Five days later, mtDNA copy number normalized to nuclear DNA was measured by qPCR; n = 5. (I) Effect of shRNA-mediated knockdown of GNPAT on OCR was measured in BAT SVF cells using a Seahorse XF24 Extracellular Flux Analyzer; n = 8. Data are expressed as mean ± SEM and were analyzed by Student’s t test. *P < 0.05.

Article Snippet: The AG diet was generated by incorporating 1 g/kg each of 1-O-hexadecyl-rac-glycerol (Chem-Impex International Inc.) and 1-O-octadecyl-rac-glycerol (Bachem) into PicoLab Rodent Diet 20.

Techniques: Western Blot, Stable Transfection, Expressing, shRNA, Confocal Microscopy